Applied Biosystems: Sequencing the Human Genome

Applied Biosystems has announced that its scientists have used human disease samples from the Baylor College of Medicine Human Genome Sequencing Center (HGSC) to sequence an entire human genome in a single run at 17-fold coverage using the SOLiD 3 System.

As part of two research projects, the SOLiD technology, equipped with bead finding and quantitation genomic analysis software, was utilized to perform two sequencing runs. The mate pair run, which contained a sample from a genetic disorder, generated 50 billion mappable bases, or 17-fold sequence coverage of the human genome, which is comprised of approximately 3 billion bases.

The second was a fragment run, which contained a sample from a brain cancer patient, and yielded 30 billion mappable bases, or 10-fold sequence coverage of the human genome.