Agilent: Automating Viral RNA Genotyping

Agilent Automation Solutions has released an applications bulletin that describes an automated system for rapid characterization of pathogens, including for example the Influenza A (H1N1) virus.

The BioCel Polymerase Chain Reaction (PCR) Viral Genotyping System provides rapid, reliable and efficient automated genetic sequencing of viruses. The system is shown to automatically sequence 40 Ribonucleic Acid (RNA) samples across 192 genes in just 11 hours. Using the company’s VWorks dynamic scheduling software, the system can sequence a minimum of 10,000 influenza viruses per year. In situations where viral outbreak reaches pandemic proportions, the throughput of the system can be doubled by running two shifts per day.

The BioCel System used in this application, comprising a variety of Agilent liquid handling options and third party devices including thermal cyclers, incubators and plate storage devices, was a dual robot system designed to optimally address the two steps of the PCR workflow.

One side of the dual robot system was for reaction preparation and clean-up, the other side for running the actual PCR reaction. The system can be extended to address other components of the workflow including the upstream nucleic acid extraction step.