Millipore has announced the introduction of three MilliTrace stem cell lines, which express Green Fluorescent Protein (GFP) under the control of various embryonic and neural stem cell markers. Due to their brilliant green glow, these cells offer scientists a quick, non-invasive way to monitor the behavior of specific stem cell populations as they proliferate, migrate, and differentiate into various lineages.
The cell lines feature GFP reporters under the control of nanog (mouse embryonic stem cells) or nestin and Sox-2 (human neural stem cells) transcription factors. The loss of these stem cell markers during differentiation causes down-regulation of the GFP, making it easy to spot cells that have not differentiated.
Following transfection with humanized mulleri GFP, every cell line is validated for high levels of GFP expression, stem cell marker expression, and multipotency. The cells are also supplied with an optimized expansion medium to help maintain expression of the transgene.
Share this: 
Del.icio.us |
Email This | 
Printer Friendly
